Effects of PTEN gene transfection on apoptosis of human bladder cancer cell lines BIU-87 / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-585464
ABSTRACT
Objective:
To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines,BIU-87,and to bring a new method of gene therapy for bladder cancer.Methods:
A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified.Expression of PTEN was detected by RT-PCR.Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry.Results:
PTEN transfected BIU-87 could steadily express PTEN mRNA,and cell apoptosis significantly increased compared with control groups.Conclusion:
Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Artigo
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