Increased expression of myeloperoxidase,CD11b and IL-8 in rat asthma model / 基础医学与临床

ABSTRACT

Objective To observe expression of myeloperoxidase(MPO),CD11b and IL-8 and to studay the functions and possible mechanism of polymorphonuclear leukocyte(PMN) in rat asthma.Methods The rats were randomly divided into two groups.Blood PMN were isolated and purified,MPO were detected by immunohistochemistry techniques,IL-8 was detected by ELISA,blood PMN CD11b were detected by flow cytometry(MFI).The total cell numbers and differential cell numbers in BALF were counted.Results MPO in bronchial wall and blood PMN,CD11b and IL-8 in blood and BALF expressions in asthma group were significantly increased than those in control group(P