Effects of long-term replacement therapy of estrogen or compound estrogen on the deposition of beta-amyloid protein in OVX rat hippocampal formation / 临床神经病学杂志

ABSTRACT

Objective To investigate the effects of long-term replacement therapy of estrogen or compound estrogen on the deposition of beta-amyloid protein (A?) in ovariectomy (OVX) rat hippocampal formation. Methods Fifty seven-month-old female Sprague-Dawley rats were randomly divided into five groups normal control, SHAM, OVX, OVX plus 17beta-estradiol (OVX/ERT), and OVX plus compound nylestriol tablet (OVX/NL). The ovariectomy was performed in OVX, OVX/NL and OVX/ERT rats and drugs were administered orally in OVX/NL and OVX/ERT rats for 35 weeks after the ovariectomy. Then the rats in all groups were sacrificed. Immunohisto-chemistry of A?, cell counting and imaging system were used to determine the change of A? deposition levels in OVX rat hippocampal formation. Results The numbers and optical density of A?-positive neurons of all hippocampal subregions and dentate gyrus in OVX rats were markedly higher than those of normal control, SHAM,OVX/NL, and OVX/ERT rats( P