Construction and characterization of hfgl 2 expression vector / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-586209
ABSTRACT
Objective:
To construct hfgl 2 expression vector(pcDNA3.1-hfgl 2) and characterize the expression of hfgl 2 in CHO cells after transfection.Methods:
Total RNA was extracted from chinese human peripheral blood monocyte cells,cDNA was obtained by reverse transcription,hfgl 2 cDNA was amplified and cloned into pcDNA3.1 and the orientation and the sequence were ensured by restriction endonucleases and sequencing assays.The recombinated plasmid was transfected into CHO cells,and the expression of hfgl 2 was detected by immunohistochemistry.Results:
A 1.3 kb long target fragment was obtained and cloned into pcDNA3.1.The orientation and sequence are correct.hfgl 2 was only expressed in those cells transfected with pcDNA3.1-hfgl 2.Conclusion:
hfgl 2 expression vector(pcDNA3.1-hfgl 2) has been successfully constructed.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Artigo
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