Expression of PPAR? in gastric carcinma tissue and effect of its ligand on human gastric carcinoma cell growth / 基础医学与临床

ABSTRACT

Objective To investigate the expression of peroxisome proliferator-activated receptor?(PPAR?) in human gastric carcinoma and the effect of its ligand 15-deoxy-△~(12,14)-Prostagliandxin J_(2)(15d-PGJ_(2)) on the growth of human gastric carcinoma MGC803 cells.Methods PPAR? and survivin mRNA were examined by RT-PCR;PPAR?,survivin,Skp2 and p27 protein were examined in cells by Western blot;proliferation was examined by MTT assay,apoptosis and cell cycle distribution were examined by flow cytometry;PPAR? protein in tissue was examined by immunohistochemistry;morphological change of apoptosis was examined by Hoechst33342 staining.Results The positive rate of PPAR? protein was significantly higher in gastric carcinomas 75.0%(40 cases) than in their paired adjacent mucosa(25.0%)((40 cases))(and)(normal)(mucosa)(20.0%(40cases)),(and)(there)(was)(no) difference between the latterones. The positive rate of PPAR?mRNA and protein were significantly higher in intestinal gastric carcinoma 95.0%(20 cases)than that in diffuse gastric carcinoma 55.0%(20 cases).Proliferation inhibition rate and apoptosis rate of MGC803 cells after_()24h were all significantly higher following 5,10,20,30 and (40 mol/L) 15d-PGJ_(2)treatment than(0 mol/L)(P