Effects of Puerarin on protein expression of hyperphosphorylated tau and ChAT in hippocampus of Alzheimer's disease rats / 临床神经病学杂志

ABSTRACT

Objective To investigate the effects of Puerarin on the expression of hyperphosphorylated-tau (P_ ser404-tau) and choline acetyltransferase (ChAT) in hippocampus of Alzheimer's disease(AD) rats.Methods The rats were divided into sham-operation group, AD group and Puerarin treated group at randomly. The AD rat models were established by injecting amyloid-beta peptide(A?_ 25-35) into right amygdala. The sham-operation rats were injected with trifluoroacetic acid in the same location. The spatial memory function was observed by Y-maze. The protein expression of P_ ser404-tau and ChAT in hippocampus were detected by immunohistochemistry.Results (1)Compared with sham-operation group,the results of maze test were down in AD group and the number of P_ ser404-tau positive cells was significantly higher,while the number of ChAT positive cells was significantly lower (all P