Increase of heme oxygenase expression in the aorta of renal hypertensive rats / 基础医学与临床

ABSTRACT

Objective To investigate the role of heme oxygenase / carbon monoxide(HO /CO) pathway in the development of renal hypertension.Methods(1)The animal model of two-kidney-one clip renal hypertensive rats were prepared and the plasma contents of angiotensin Ⅱ(AngⅡ) were determined by radiommunoassay.(2)The activity of HO was tested at 2,4,6,8 weeks after surgery.(3) Western blot was used to estimated the level of HO-1 expression of aorta.Results In 2K1C rats,there was a significant increase in blood pressure at 2K1C.At the same time,rennin-agiotensin system(RAS) in plasma was activated and the content of AngⅡ obviously increased.The activity of HO was greater at 4-and 6-week in 2K1C group than sham group(P