Comparative Analysis of Human Epidermal and Peripheral Blood gammadelta T Cell Cytokine Profiles
Annals of Dermatology
;
: 308-313, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-58744
ABSTRACT
BACKGROUND:
Human epidermal gammadelta T cells are known to play crucial roles in the defense and homeostasis of the skin. However, their precise mechanism of action in skin inflammation remains less clear.OBJECTIVE:
In this study, we analyzed the cytokine expression profile of human epidermal gammadelta T cells and compared it to that of peripheral blood gammadelta T cells to investigate the specific activity of epidermal gammadelta T cells in modulating skin inflammation.METHODS:
We isolated gammadelta T cells from epidermal tissue or peripheral blood obtained from healthy volunteers. Isolated gammadelta T cells were stimulated using immobilized anti-CD3 antibody and interleukin-2 plus phytohaemagglutinin, and were then analyzed using a cytokine array kit.RESULTS:
Both epidermal and peripheral blood gammadelta T cells produced comparable levels of granulocyte-macrophage colony-stimulating factor, I-309, interferon-gamma, macrophage migration inhibitory factor, macrophage inflammatory protein-1alpha, and chemokine (C-C) ligand 5. The epidermal gammadelta T cells produced significantly higher levels of interleukin-4, -8, -13, and macrophage inflammatory protein-1beta than the peripheral blood gammadelta T cells did. Notably, the epidermal gammadelta T cells produced several hundred-fold higher levels of interleukin-13 than interleukin-4.CONCLUSION:
These results suggest that the epidermal gammadelta T cells have a stronger potential to participate in the Th2-type response than the peripheral blood gammadelta T cells do. Furthermore, epidermal gammadelta T cells might play an important role in the pathogenesis of Th2-dominant skin diseases because of their active production of interleukin-13.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pele
/
Dermatopatias
/
Linfócitos T
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Interleucina-4
/
Interferon gama
/
Interleucina-2
/
Interleucina-13
/
Epiderme
/
Voluntários Saudáveis
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2014
Tipo de documento:
Artigo
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