Establishment of human mesangial cell line with high expression of type A scavenger receptor and its function / 基础医学与临床
Basic & Clinical Medicine
;
(12)2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-587673
ABSTRACT
Objective To determine the function and binding properties of SR-A in HMC.Methods A human mesangial cell line(HMCL) with high expression of type A SCR(HMCL-SCR) was established after stable transfection of expressive vector with cDNA encoding SR-A.Uptake of fluorescence Dil-labeled acetylated low density lipoprotein(Dil-Ac-LDL) and oxidized LDL(Ox-LDL) by HMC were evaluated by fluorescence microscopy,con-focal microscopy and Oil Red "O" staining respectively.SCR mRNA expression was examined with reverse transcription-polymerase chain reaction(RT-PCR).Results More uptake of Ox-LDL and Ac-LDL was observed in HMCL-SCR than that in the untransfected HMCL.Unlabeled Ox-LDL and Ac-LDL at a 40-fold excess concentration competed significantly with the uptake of Dil-Ac-LDL by HMC.Otherwise this phenomenon was not observed when co-cultured with LDL.Conclusion This results suggest that SR-A mediates the uptake of modified-LDL with high affinity and high specificity in human mesangial cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Basic & Clinical Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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