Effect of morphine on gene expression of enzymes related to purine nucleotides metabolism in PC12 cells / 吉林大学学报(医学版)

ABSTRACT

Objective To study the effect of morphine on gene expression of enzymes involved in salvage and catabolism pathways of purine nucleotides metabolism in nerve cells.Methods PC12 cells were cultivated and divided into morphine treated groups which were treated with morphine(10 mg?L~(-1) culture) for 12,24,48, 72 h,and control groups which were treated with normal saline for 12,24,48 and 72 h.Total RNA of PC12 cells was isolated.HGPRT,AK,ADK mRNA levels were determined by using the reverse transcription polymerase chain reaction(RT-PCR); the ?-actin mRNA expression was used as an internal control.Results As compared with corresponding control groups,HGPRT mRNA levels in PC12 cells were increased significantly after 12 and 24 h treatment with morphine(P