Hypoxia enhanced proliferation of C_2C_(12) cells and its possible mechanism / 医学研究生学报

ABSTRACT

Objective:To explore the role of hypoxia in C_2C_(12) cells proliferation.Methods:We chose 10% oxygen concentration.Cell numbers were counted with a hemacytometer.The proliferous indexes were observed with a flow cytometer.HIF-1? protein was determined by Western blot,mRNA expression was determined with reverse transcriptase-polymerase chain reaction(RT-PCR).Results:The cell numbers and proliferous indexes were higher(P