Screening of proteins interacting with neuropeptide CART / 基础医学与临床

ABSTRACT

Objective The objective was to look for cocaineand amphetamine-regulated transcript peptide(CART) receptors or proteins interacted with CART.Methods A cDNA library from rat brain was constructed.Then the BacterioMatch two-hybrid system was used to screening for proteins interacted with CART by using CART41-102 as bait.Results The cDNA library and bait pBT-CART41-102 were constructed successfully.The library harvested 3.37?106 individuals,and the reconstruction rate was 98.5%.5.01?106 cotransformed clones were screened,93 clones were conformtively identified to interacting with CART and were DNA sequenced.By bioinformation analysis,6 known proteins interacted to CART were identified.Other unknown interaction proteins with short exactly similiar motif to 22 membraneproteins or receptors were also identified.Conclusion CART might interact to proteins for energy balance,cell proliferation and enzymes related to protein processing and degrading.