Differentially expressed gene in rat hippocampus irradiated by 6MV X-rays / 医学研究生学报

ABSTRACT

Objective: To observe differentially expressed genes in rat hippocampus irradiated by 6 MV X-rays during radiotherapy.Methods:After whole brain irradiation of 50 Gy in 25 fractions with 2 Gy per fraction,the capability of learning and memory was investigated in a shuttle box model,then rats were killed and the hippocampus were taken,mRNA from sham irradiation group and irradiation group were reversely transcribed to cDNA with incorporation of fluorescence dUTP(Cy5 and Cy3 dUTP) to prepare for hybridization probes.The mixed probes were hybridized to the cDNA microarray.Axon Genechix 4000 BandGenePix Pro3.0 software was used to scan and analyze the fluorescent signals. Results:①The active avoidance response of irradiated rats were lowered significantly.②Compared with the sham group,significant changes took place in 43 genes,among which 28 genes were upregulated and 15 down regulated,other genes did not change significantly.③Among the 43 malexpressed genes,16 were reported previously,others were newly found genes. Conclusion: The influence of ionizing radiation on learning and memory was a complex course,including many transduction factors and might be related to mitochondrial dysfunction.