Induction of Interleukin-22 (IL-22) production in CD4+ T Cells by IL-17A Secreted from CpG-Stimulated Keratinocytes
Annals of Dermatology
;
: 579-585, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-59031
ABSTRACT
BACKGROUND:
Interleukin-17A (IL-17A) is mainly secreted from Th17 cells that are activated by various stimuli including CpG oligodeoxynucleotide, a Toll-like receptor 9 (TLR9) ligand. Recently, it has been demonstrated that keratinocytes play an important role in the pathogenesis of psoriasis.OBJECTIVE:
To investigate the potential role of keratinocytes, we examined whether TLR9 ligand CpG induces IL-17A expression in keratinocytes.METHODS:
We used HaCaT keratinocytes as a model system, and determined CpG-induced IL-17A using enzyme-linked immunosorbent assay and Western blot.RESULTS:
When HaCaT keratinocytes were treated with CpG, the expression of several cytokines including IL-17A, tumor necrosis factor-α and CCL20 was markedly increased. Treatment with nuclear factor (NF)-κB inhibitor significantly blocked the CpG-induced IL-17A production, indicating that CpG induced IL-17A expression through the NF-κB signaling pathway. In addition, IL-17A secreted from keratinocytes stimulated the CD4⁺ T cells, resulting in strong induction of IL-22 production.CONCLUSION:
Since IL-22 is an important mediator for psoriatic inflammation, our data suggest that keratinocytes can participate in the pathogenesis of psoriasis via the TLR9-dependent IL-17A production.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Psoríase
/
Ensaio de Imunoadsorção Enzimática
/
Linfócitos T
/
Queratinócitos
/
Western Blotting
/
Citocinas
/
Interleucina-17
/
Receptor Toll-Like 9
/
Células Th17
/
Inflamação
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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