Comparison between two isolation methods of human mesenchymal stem cells in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-590604
ABSTRACT
AIM:
There is no standard isolation method of mesenchymal stem cells.Percoll density gradient centrifugation is treated as the traditional method among numerous methods but it is so complicated.This study compares whole bone marrow culture to traditional Percoll density gradient centrifugation in order to establish a better method in vitro.METHODS:
Experiments were conducted at Central Laboratory of Affiliated Hospital of Qingdao University Medical College from September 2006 to June 2007.Bone marrow was supported by patients who had autologous stem cell transplantation by endocrinology department for diabetes treatment.The experiments had known by the patients and authorized by the hospital ethic committee.The two different methods,whole bone marrow culture and Percoll density gradient centrifugation,were compared in the number of cell clone,cell morphology,cell superficial mark,the differentiation into fat cell.RESULTS:
① The number of clone cell by whole bone marrow culture was more than by Percoll density gradient centrifugation(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2007
Tipo de documento:
Artigo
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