RPTC cells toxicity induced by cisplatin and anti-apoptosis mechanism of BCL-2 / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
em Zh
| WPRIM
| ID: wpr-590835
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WPRO
ABSTRACT
Objective To explore the mechnism of toxicity induced by cisplatin on renal proximal tubular cells(RPTC) line and anti-apoptosis mechanism of BCL-2 with transfection of different mutant BCL-2 in vitro.MethodsRPTC was translocated with different mutants BCL-2(s).Cell apoptosis induced by cisplatin on RPTC was analyzed with confocal and flurencent microscope.The cell apoptosis was measured with Hoechst33258 after treatment with cisplatin.Results Different mutant BCL-2(BCL-acta,BCL-cb5)were translocated on mitochondrial and Endoplasmic Reticulum(ER) respectively.BCL-acta protected RPTC from apoptosis induced by cisplatin more easily than BCL-cb5 group in a time-dependant manner(P
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Zh
Revista:
Basic & Clinical Medicine
Ano de publicação:
2006
Tipo de documento:
Article