Preparation and in vitro antioxidative activity of acetylated fucoidan extracted from Laminaria japonica / 中国海洋药物

ABSTRACT

Objective To explore the optimum method for preparation of acetylated fucoidan extracted from Laminaria japonica,and test the antioxidative activity of acetylated fucoidans prepared under different conditions in vitro.Methods Using acetic anhydride as acetylated reagent,N-Bromosuccinimide(NBS) as catalyst,the effects of reaction time,temperature and volume of acetylated reagent were tested by the orthogonal design method.The antioxidative activity of the prepared acetylated fucoidans in vitro was determined,including scavenging activity against superoxide,hydroxyl and DPPH radical and reducing power.Results Volume of acetylated reagent and reaction temperature were the significant factors(P