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The Change of Podocyte beta-Catenin by Puromycin Aminonucleoside
Journal of the Korean Society of Pediatric Nephrology ; : 138-145, 2011.
Artigo em Coreano | WPRIM | ID: wpr-59141
ABSTRACT

PURPOSE:

To test whether the expression of beta-catenin, a component of podocyte as a filtration molecule, would be altered by puromycin aminonucleoside (PAN) in the cultured podocyte in vitro.

METHODS:

We cultured rat glomerular epithelial cells (GEpC) with various concentrations of PAN and examined the distribution of beta-catenin by confocal microscope and measured the change of beta-catenin expression by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR).

RESULTS:

We found that beta-catenin relocalized from peripheral cytoplasm to inner cytoplasm, therefore, intercellular separations were seen in confluently cultured cells by high concentrations of PAN in immunofluorescence views. In Western blotting of GEpC, PAN (50 microg/mL) decreased beta-catenin expression by 34.9% at 24 hrs and 34.3% at 48 hrs, compared to those in without PAN condition (P<0.05). In RT-PCR, high concentrations (50 microg/mL) of PAN also decreased beta-catenin mRNA expression similar to protein suppression by 25.4% at 24 hrs and 51.8% at 48 hrs (P<0.05).

CONCLUSION:

Exposure of podocytes to PAN in vitro relocates beta-catenin internally and reduces beta-catenin mRNA and protein expression, which could explain the development of proteinuria in experimental PAN-induced nephropathy.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteinúria / Ácido Ascórbico / RNA Mensageiro / Puromicina / Puromicina Aminonucleosídeo / Células Cultivadas / Western Blotting / Imunofluorescência / Citoplasma / Células Epiteliais Limite: Animais Idioma: Coreano Revista: Journal of the Korean Society of Pediatric Nephrology Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteinúria / Ácido Ascórbico / RNA Mensageiro / Puromicina / Puromicina Aminonucleosídeo / Células Cultivadas / Western Blotting / Imunofluorescência / Citoplasma / Células Epiteliais Limite: Animais Idioma: Coreano Revista: Journal of the Korean Society of Pediatric Nephrology Ano de publicação: 2011 Tipo de documento: Artigo