Study of Model of Single Pedicle Separaration Flap / 医疗卫生装备

ABSTRACT

Objective To design a new flap model using for study on effects of some drugs which would maximize flap survival,and to evaluate its experimental availability.Methods The Wistar rat was used as the experimental animal.On the back of each rat,a skin flap,measuring 1.5 cm ? 6 cm,caudally based,was elevated and separated.Wound edges and the flap bed were separated from the flap by a silicone sheet and the drug was put in the areas between flap and sheet.On the seventh day after the flap elevation,historical and physiological measurement to blood flow and skin specimens would be done.The repeatability of the model was calculated.Results Single pedicle separaration flap needed 26 rats as samples when the resolution was 10% in testing,while the back flap model(10 cm ? 3 cm ) needed 28 rats.The needed samples were 2 rats and 5 rats respectively when the resolution was 25%.So it was believed that with high repeatability,the model of pedicle separation flap met the basic principle of testing design and was suitable for the study of the same kind.Conclusion The model can be used with confidence to study the effects of drugs on the flap survival.