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Construction of a lentiviral expression vector that inhibits enhanced green fluorescent proteins / 医学研究生学报
Journal of Medical Postgraduates ; (12)2003.
Artigo em Chinês | WPRIM | ID: wpr-591972
ABSTRACT

Objective:

To construct a lentiviral expression vector that inhibits enhanced green fluorescent proteins(EGFP).

Methods:

The EGFP shRNA was cloned into the pLL3.7 lentivirus vector digested with XbaⅠ/XhoⅠ.J558L and Hela cells were infected by lentiviruses,and the EGFP expression was observed with the fluorescence microscope.

Results:

The EGFP RNAi sequence was correctly cloned into the lentivirus vector with the U6 promoter,and the EGFP expressions of Hela and J558L cells were suppressed after infected by lentivirus containing EGFP shRNA.

Conclusion:

The lentivirus vector effectively inhibited EGFP expression,which could be used to mediate further researches on gene function.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Medical Postgraduates Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Medical Postgraduates Ano de publicação: 2003 Tipo de documento: Artigo