Analysis of Toxoplasma gondii Specific Antigens by Two Dimensional Electrophoresis and Western Blotting / 中国寄生虫学与寄生虫病杂志

ABSTRACT

The total protein of Toxoplasma gondii RH strain tachyzoites was separated by two-dimensional electrophoresis (2-DE), and Western blotting was performed to find out distinct antigens. 209 spots were detected through Coomassie brilliant blue-stained small gels (7?8 cm, pH 3-10). Western blotting showed 17 specific antigen spots with sera of the experimental group and two nonspecific spots with the control sera.