Tamoxifen inhibits cell proliferation and currents of sodium channel in glioma cell line SHG-44 / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
em Zh
| WPRIM
| ID: wpr-592583
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the effects of tamoxifen on the proliferation of SHG-44 glioma cells and the currents of sodium channel. Methods The cell activity was detected by MTT. The alteration of cellular proliferation and apoptosis were dectected by flow cytometer. Whole-cell patch clamp technique was used to record the Na currents.Results After treatment with tamoxifen,the cells began aging and shedding and cell counting decreased.The cells in G2/M cell cycle were more than that in control and the apoptosis ration increased. Tamoxifen significantly decreased the amplitude of Na currents of SHG-44 cell line.This blocking effect was dose-dependent and voltage-dependent.When the holding potential was 0 mV, 8 ?mol/L tamoxifen could block this currents by 69%.The half inhibition concentration(IC50) was 5.54 ?mol/L. Conclusion Tamoxifen can inhibit SHG-44 glioma cells proliferation.The inhibion of sodium channel may be one of its mechanisms.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Basic & Clinical Medicine
Ano de publicação:
2006
Tipo de documento:
Article