Study on extraction techniques of dietary fiber from Undaria pinnatifida / 中国海洋药物

ABSTRACT

Objective To extract dietary fiber from Udaria pinnatifida by using enzymatic-chemical extraction method.Methods All the factors that affect the extraction rate are enzyme content,enzymolysis temperature,enzymolysis time,Na2CO3concentration,Na2CO3extraction temperature,Na2CO3content and extraction time.They were tested with orthogonal experiment.Results The optimal conditions for extraction were determined as followsthe material was hydrolyzed at 50℃ for 1.5h with protease and cellulase mixed at the ratio of 30∶1,followed by the extraction at 65℃ for 3h with 30 volumes of 1% Na2CO3 solution.Under these conditions,the extraction rate could be as high as 12.65%with the color near light yellow.It could be used as additive source of dietary fiber for functional foods.Conclusion The optimum conditions for extraction dietary fiber were established.