Inhibiting Cytochrome C Oxidase Leads to Alleviated Ischemia Reperfusion Injury
Korean Circulation Journal
;
: 193-200, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-59345
ABSTRACT
BACKGROUND AND OBJECTIVES:
The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways. MATERIALS ANDMETHODS:
We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial H2O2 and mitochondrial complex activity.RESULTS:
KCN pre-treatment led to decreased infarction area after IR injury and improved cardiac function. KCN pre-treated group challenged with IR injury was associated with reduced ROS production through inhibition of activity and not downregulation of CcO expression. In addition, KCN pre-treatment was associated with enhanced expression and activity of mitochondrial antioxidase, suggesting the role of CcO in regulating IR injury through oxidative stress.CONCLUSION:
KCN pre-treatment reduced the severity of IR injury. The potential mechanism could be increased endogenous anti-oxidase activity and consequently, the enhanced clearance of ROS.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cianeto de Potássio
/
Traumatismo por Reperfusão
/
Regulação para Baixo
/
Espécies Reativas de Oxigênio
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Estresse Oxidativo
/
Citocromos
/
Citocromos c
/
Infarto
/
Isquemia
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Circulation Journal
Ano de publicação:
2017
Tipo de documento:
Artigo
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