Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii
The Korean Journal of Parasitology
;
: 251-254, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-59371
ABSTRACT
Monoclonal antibody (mAb) Tg786 against Toxoplasma gondii has been found to detect a 42-kDa rhoptry protein (ROP6) which showed protease activity and host cell binding characteristics after secretion. Using the mAb, a colony containing a 3'-UTR was probed in a T. gondii cDNA expression library. A full length cDNA sequence of the rhoptry protein was completed after 5'-RACE, which consisted of 1,908 bp with a 1,443 bp ORF. The deduced amino acid sequence of ROP6 consisted of a polypeptide of 480 amino acids without significant homology to any other known proteins. This sequence contains an amino terminal stop transfer sequence downstream of a short neutral sequence, hydrophilic middle sequence, and hydrophobic carboxy terminus. It is suggested that the ROP6 is inserted into the rhoptry membrane with both N- and C-termini.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeo Hidrolases
/
Toxoplasma
/
Dados de Sequência Molecular
/
Biblioteca Gênica
/
Proteínas de Protozoários
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Regiões 3' não Traduzidas
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2006
Tipo de documento:
Artigo
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