Comparison of biological characterisitics between adult and fetal bone marrow mesenchymal stem cells

Xiaojuan SUN; Nailong YANG

ABSTRACT

BACKGROUND:

At present,mesenchymal stem cells(MSCs) is mainly obtained from stem cells.But the quantity of human bone marrow mesenchymal stem cells(BMSCs) is lower.Fetal MSCs possess weak immunogenicity and good differentiation potential.

OBJECTIVE:

To compare the biological difference in adult and fetal BMSCs.DESIGN,TIME AND

SETTING:

The cytology in vitro controlled study was performed at the Central Laboratory,Hospital Affiliated to Medical College,Qingdao University from February to October 2008.MATERIALSThree samples of adult bone marrow blood were obtained from Hospital Affiliated to Medical College,Qingdao University.Three samples of fetal bone marrows were supplied by Qingdao Municipal Hospital.

METHODS:

Human and fetal BMSCs were harvested in vitro by the Percoll method,and incubated in LG-DMEM containing 10% volume fraction fetal bovine serum.Till cells were 90% confluent,cells were digested and passaged.The same passage of adult and fetal BMSCs were collected,and treated with KGla suspension and DMEM containing fetal bovine serum at 37 ℃ in 0.05 volume fraction CO2 for 2 hours.Non-adherent cells were removed.At the third passage,BMSCs at a density of 1?106 were incubated.When cells were 60%-80% confluent,cells were incubated in osteoblast medium for 16 days.MAIN OUTCOME

MEASURES:

Cell morphological changes were measured.Growth curve was detected by MTT assay.Cell adherent rate in vitro was calculated.Alkaline phosphatase staining was used to detect the differentiational results of osteoblasts.

RESULTS:

Adult and fetal BMSCs showed spindle-shape,but the fetal BMSCs were small.The doubling time of them was 50 hours and 30 hours,respectively.Compared with the adult BMSCs,cell adherent rate was significantly lower in the fetal BMSCs(t=4.22,P