Promotive effect of basic fibroblast growth factor on cat corneal endothelial cell proliferation

Wenjuan LUO; Jing LIN; Chuanfu WANG

ABSTRACT

BACKGROUND:

Previous experiments reported that basic fibroblast growth factor(bFGF) can change the shape of corneal endothelial cells,accelerate cell division and chemotaxis,and repair corneal endothelia cells injury in vivo and in vitro.

OBJECTIVE:

To investigate the effect of bFGF on cat corneal endothelial cells proliferation. DESIGN,TIME AND

SETTING:

The cell morphological observation was performed at the Central Laboratory of the Affiliated Hospital of Medical College of Qingdao University between January 2005 and December 2006. MATERIALSDomestic cats were purchased from Experimental Animal Center of the Affiliated Hospital of Medical College of Qingdao University.

METHODS:

The corneal endothelial cells of cats were primarily cultured,and neurone specific enolase(NSE) immunocytochemistry staining was performed. After cell passage,bFGF(1,10,100 ?g/L) was added,continuously hatched for 1-5 days. MAIN OUTCOME

MEASURES:

MTT method was used to determine the proliferation of corneal endothelial cells;meantime,inverted phase contrast microscope and transmission electron microscope were used to observe the ultrastructure of cells.

RESULTS:

At days 1,3 and 5 after bFGF was added,the absorbance value in 490 nm was significantly higher than that of control group,especially in 10 ?g/L group.

CONCLUSION:

The bFGF can promote proliferation of cat corneal endothelial cells with effective concentration of 10 ?g/L .