Effect of hepatitis B virus X protein on nuclear localization and transcription of ?-catenin in human liver cell / 基础医学与临床

ABSTRACT

Objective To investigate the interaction between HBx protein and ?-catenin for exploring molecular mechanism in HBV-associated hepatocellular carcinoma.Methods Iimmunocytochemistry and Luciferase Assay were used to detect the endocellular location and transcriptional activity of ?-catenin in normal liver cell line L02 infected by Ad-HBx.Results Immunocytochemistry there was markedly increased expression of ?-catenin in cytoplasm and nucleus.Luciferase Assay Ad-HBx led to a significant increase of TCF-4-dependent transcription,the TOP count of Ad-GFP and Ad-HBx-GFP was 1.4 fold and 11 fold as compared to the negative control respectively(P