Physicochemical properties of xenogeneic deproteinized cancellous bone as stent materials

Ge SUN; Chunyang GAO; Bin ZHOU

ABSTRACT

BACKGROUND:

Deproteinized cancellous bone can be used as a new kind of bone graft material due to its wide resources andbiological characteristics if the problem of immunogen can be solved.

OBJECTIVE:

To study the properties of deproteinized cancellous bone following preparation with physico-chemical methods,in addition,to explore the feasibility of xenogeneic deproteinized cancellous bone used as scaffolds in bone tissue engineering. DESIGN,TIME AND

SETTING:

A comparison observation was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy between February 2008 and January 2009. MATERIALSDeproteinized bone was derived from pigs and goals femoral cancellous bone followed by treating with 11 methanol extract and 30% H2O2.

METHODS:

The mechanical properties of vector were examined by scanning electron-microscopy,X-rays diffraction analysis,differential scanning calorimetry,and mechanical experimental instrument. The density of third passage of human bone mesenchymal stem cells (MSCs) of iliac bone was regulated to 2?108/L,vaccinated to vector,and the cell adhesion and proliferation was observed by inverted microscope and scanning electron microscope. Forty BALB/C mice were randomly dividedinto the experimental and control groups,with 20 animals in each group. Muscle behind femur was made muscle pouch,which was implanted pig deproteinized bone in the experimental group,and no other treatment in the control group. The level of serumcarnine,glutamic-oxal(o)acetic transaminase (GOT),as well as glutamic-pyruvic transaminase (GPT) were measured at weeks 12,4,8 after operation. Meanwhile,the immunogenicity of pig deproteinized bone and specimen of deproteinized bone in mice were detected at the 4 weeks after implantation. MAIN OUTCOME

MEASURES:

Physicochemical properties and immunogenicity of deproteinized bone were analyzed by in vitroexperiment. And the bio-safety was evaluated by animal experiment.

RESULTS:

The inorganic composition of deproteinized cancellous bone was hydroxyapatite ceramic,which is identical with nature composition of human bone. It showed that deproteinized cancellous bone maintained natural network pore system and kept good mechanical property. The MSCs grew well on deproteinized cancellous bone due to its low immunogenicity and toxicity.

CONCLUSION:

The xenogeneic deproteinized cancellous bone is an excellent vector in bone tissue engineering.