Establishment and characterization of a mouse medulloblastoma cell line / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
em Zh
| WPRIM
| ID: wpr-595290
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the molecular mechanism of medulloblastoma.Methods The cell line with PARP-1 and P53 null mutation was established and characterized.Mouse medulloblastoma cell line derived from PARP-1/P53 double knockout mice was established.We analyzed cell characters after 30 passages,using neuronal cell-specific markers by immunofluorescence.The cells were transfected with pEGFP-C1-Hparp-1 and pEGFP-C1 plasmids,the expression of PARP-1 protein was detected by immunofluorescence stainning and Western blot.Results The cells showed positive immunoactivity for the neuronal-specific markers such as Vimentin,Dcx and ?Ⅲ-Tubulin,and cells were negative for PARP-1 protein.Exogenous PARP-1 expression was visualized by immunofluorescence and Western blot analysis after pEGFP-C1-Hparp-1 transfection.Conclusion Mouse medulloblastoma cell line with defective function for DNA damage recovery has been successfully established,which provides a useful tool for further dissecting the molecular mechanism and pathogenesis of medulloblastoma.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Basic & Clinical Medicine
Ano de publicação:
2006
Tipo de documento:
Article