Interactions of Helicobacter pylori and its L-forms with surfactant protein D / 基础医学与临床

ABSTRACT

Objective To explore the affinify of combination of Helicobacter pylori (Hp) and its L-form with surfactant protein D(SP-D),and to find function of SP-D in Hp infection and immunity. Methods SP-D was extracted from the lung of rats by Tris-HCl-EDTA and maltose-agarose affinity column. The combination of Hp and SP-D was detected by slide and tube agglutination tests,and the agglutination inhibition with SP-D immune serum was carried out. The L-form of Hp was induced by Ceftriaxon sodrum and identified by PCR and sequencing of the 16S rDNA fragment.The combination of SP-D and the L-form was tested by slide agglutination test. Results Agglutination of Hp NCTC11637 and SP-D was observed on slide,but NCTC11639,SS1 and the L-form failed to agglutinate. Agglutination was found 45 minutes after reaction and was inhibited by SP-D immune sera. Conclusion The ability of combination with SP-D was different between strains of Hp.The combination could be weakened or vanished when Hp lost the cell wall.