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Adipose Gene Expression Profiles Related to Metabolic Syndrome Using Microarray Analyses in Two Different Models
Diabetes & Metabolism Journal ; : 356-365, 2014.
Artigo em Inglês | WPRIM | ID: wpr-59599
ABSTRACT

BACKGROUND:

Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-gamma agonists and increased in OLETF rats using microarrays in two different models.

METHODS:

In the first microarray analysis, PPAR-gamma agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats).

RESULTS:

Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-gamma agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count.

CONCLUSION:

Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-gamma agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Homologia de Genes / Proteínas de Transporte / Índice de Massa Corporal / Reação em Cadeia da Polimerase / Interleucina-6 / Adipócitos / Ratos Endogâmicos OLETF / Peroxissomos / Modelos Animais / PPAR gama Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Inglês Revista: Diabetes & Metabolism Journal Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Homologia de Genes / Proteínas de Transporte / Índice de Massa Corporal / Reação em Cadeia da Polimerase / Interleucina-6 / Adipócitos / Ratos Endogâmicos OLETF / Peroxissomos / Modelos Animais / PPAR gama Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Inglês Revista: Diabetes & Metabolism Journal Ano de publicação: 2014 Tipo de documento: Artigo