Methodological research on the application of AFLP in complicated samples / 中国基层医药

ABSTRACT

Objective To explore the application probability of AFLP method (Amplified Fragment Length Polymorphism) for the genetic study of complicated samples and to make clear the analytical approach on polymorphic fragments.Methods An AFLP fragement sequence,resolved between murines with high and low metastatic hepatocarcinoma,was retrieved from a reference paper and was annotated on NCBI database by different BLAST programs. Results The retrieved information varied along with the different BLAST programs, nevertheless BLASTX program provided more comprehensive information and showed that the differentiated fragment exhibited high homology with terminase large subunits from Burkholderia phage Bups phil and Mycobacterium tuberculosis H37Ra. However, there is no detailed information for the latter,key words search returned the relationship between terminase and the formation and metastasis of tumors and provided the further research clues. Conclusions Whole genome screening in parallel of AFLP markers between genetically differentiated paired materials would readily produce informatic fragments. Further analysis of the sequenced fragments by informatic methods would facilitate the subsequent research.