Expressions of myocardial ?-MHC and ?-MHC mRNA in rats with pressure overload / 医学研究生学报

ABSTRACT

Objective: Recent years has witnessed new progress in the molecular genetic study of the relationship between the myosin heavy chain and myocardial hypertrophy.This study aimed to observe the expressions of myocardial ?-myosin heavy chain(?-MHC) and ?-myosin heavy chain(?-MHC) mRNA in rats with pressure overload,and to investigate the mechanism by which abdominal aorta constriction induces left ventricular hypertrophy.Methods: Male SD rats were randomly divided into a sham operation and model group.The models were made by clamping the suprarenal abdominal aorta with a silver chip.Eight weeks later,changes in the left ventricular mass index(LVMI) and the ultra microstructure of the left ventricular myocyte were observed,and the expressions of ?-MHC and ?-MHC in the myocardial tissue were detected by RT-PCR.Results: Compared with the sham operation group,the model rats showed markedly increased LVMI(P