Western blot of the expression of J chain-human netrophil peptide-1 / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-596470
ABSTRACT
Objective:
Defensins from human neutrophils exhibit a broad antimicrobial activity.The transfection of the gene fused by human neutrophil peptide 1(HNP1) with the J chain into other cells may induce the in vitro expression of HNP1 to play antimicrobial roles.The authors analyzed the influencing factors on the expressed proteins of HNP1 and the J chain gene in COS-7 cells.Methods:
Western blot was used to analyze the technical factors influencing the HNP1 protein expression.Results:
Recombinant J-HNP1 was expressed in the cells successfully.Conclusion:
The expression of J-HNP1 can be detected inside and outside the cells by Western blot.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS