Sample related factors affecting short-term culture of erythrocytic Plasmodium vivax in vitro / 中国血吸虫病防治杂志

ABSTRACT

Objective To explore the sample reIated factors affecting the short-term culture of erythrocytic Plasmodium dvax in vitro.Methods The vivax malaria blood samples were collected from the patients with malaria in endemic areas,and then incubated with McCoy's 5A medium in an incubator containing 5%CO_2 at 37℃.The factors affecting the short-term culture of Plasmodium vivax were analyzed.Results Plasmodium vivax could finish one asexual cycle in the selected medium.By analyzing the culture results of 74 samples.it was found that the factors affecting the short-term culture included long time delaying al room temperature(>4 h),single stage(only parasites in ring stage were found),patients taking antimalarials,antibiotics or sulfonamides.and low parasitemia.Conclusion The sample related factors are important to the short-term culture of erythrocytic Plasmodium vivax in vitro.