Expression,purification and biological activity assays of global human C1 q and tumor necrosis factor related protein-2 / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 1023-1025,1026, 2014.
Article
em Zh
| WPRIM
| ID: wpr-599304
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WPRO
ABSTRACT
Aim To prepare soluble global human C1 q and tumor necrosis factor related protein-2 in Escherichia coli. Methods Recombinant expression plasmid was transformed into strain BL21-codonplus (DE3),and the recombinant protein of Trx-gH2 was expressed by IPTG induction and then purified by Ni-NTA affinity and gel filtration chromatography.Results The purified recombinant Trx-gH2 was shown to be active under in vi-vo and in vitro assay conditions.Conclusion Active recombi-nant global hCTRP2 is efficiently prepared from Escherichia coli protein expression system.
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Zh
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2014
Tipo de documento:
Article