Silibinin Inhibits LPS-Induced Macrophage Activation by Blocking p38 MAPK in RAW 264.7 Cells
Biomolecules & Therapeutics
; : 258-263, 2013.
Article
em En
| WPRIM
| ID: wpr-59935
Biblioteca responsável:
WPRO
ABSTRACT
We demonstrate herein that silibinin, a polyphenolic flavonoid compound isolated from milk thistle (Silybum marianum), inhibits LPS-induced activation of macrophages and production of nitric oxide (NO) in RAW 264.7 cells. Western blot analysis showed silibinin inhibits iNOS gene expression. RT-PCR showed that silibinin inhibits iNOS, TNF-alpha, and IL1beta. We also showed that silibinin strongly inhibits p38 MAPK phosphorylation, whereas the ERK1/2 and JNK pathways are not inhibited. The p38 MAPK inhibitor abrogated the LPS-induced nitrite production, whereas the MEK-1 inhibitor did not affect the nitrite production. A molecular modeling study proposed a binding pose for silibinin targeting the ATP binding site of p38 MAPK (1OUK). Collectively, this series of experiments indicates that silibinin inhibits macrophage activation by blocking p38 MAPK signaling.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosforilação
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Sítios de Ligação
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Modelos Moleculares
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Expressão Gênica
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Trifosfato de Adenosina
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Western Blotting
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Fator de Necrose Tumoral alfa
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Silybum marianum
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Sistema de Sinalização das MAP Quinases
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Proteínas Quinases p38 Ativadas por Mitógeno
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biomolecules & Therapeutics
Ano de publicação:
2013
Tipo de documento:
Article