Determination of Oxidized Glutathione in Glutathione Tablets by HPLC / 中国药师

ABSTRACT

Objective:To establish an HPLC method for the determination of oxidized glutathione in glutathione tablets. Meth-odsAnAgilentTC-C18(250mm×4.6mm,5 μm)columnwasusedwiththemobilephaseofphosphatesolution(pH3.0)-methanol (96∶4). The flow rate was 1. 0 ml·min-1. The detection wavelength was 210 nm. The column temperature was 30℃ and the injec-tion volume was 20 μl. Results:The linear range of oxidized glutathione was 1.403-22.450 μg·ml-1(r=0.999 9). The recovery was 98. 9% with RSD of 1. 5%(n=9). Conclusion:The method is accurate and repeatable, and can control the content of oxidized glutathione in glutathione tablets effectively. .