Determination of Berberine Hydrochloride in Shenshe Ointments by HPLC / 中国药师
China Pharmacist
;
(12): 1576-1577,1578, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-599706
ABSTRACT
Objective:
To establish the determination method for berberine hydrochloride in Shenshe ointments.Methods:
The quantitative analysis of berberine hydrochloride in Shenshe osintments was carried out by HPLC with a Kromsal C18 chromatographic column (250 mm × 4. 6 mm, 5 μm), the mobile phase was acetonitrile-0. 1% phosphoric acid (49∶51) (adding 0. 2g sodium dode-cylsulphate into 100 ml solution) with the flow rate of 1. 0 ml·min-1 and the detection wavelength of 320nm, the temperature of col-umn was room temperature, and the injection volume was 20 μl.Results:
The linear range was 0. 059 2-0. 296 0 g·L-1 with good correlation(r=0. 999 4). The average recovery was 99. 80% and RSD was 0. 24%(n=6).Conclusion:
The established method is accurate, specific and reproducible, and suitable for the determination of berberine hydrochloride in Shenshe ointments.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2014
Tipo de documento:
Artigo
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