Expression of autophagy associated proteins in acute lung injury induced by remote limb ischemia reperfusion in rats / 重庆医学

ABSTRACT

Objective To explore the early expression of autophagy associated proteins in lung tissues in acute lung injury in‐duced by remote limb ischemia reperfusion(LIR‐ALI)in Rats .Methods Twelve adult male SD rats weighting 220-250 g were made LIR‐ALI models and divided randomly into two groups(6 in each group)Sham operation group and ischemia‐reperfusion(I/R)group .The rats were anesthetized and the tissues of lung were removed at the end of 4 hours of reperfusion after 3 hours of is‐chemia .The serum lactate dehydrogenase(LDH) were detected with ELISA and the pathological changes of lung tissues were ob‐served by immunofluorescence techniques ;the expression of Beclin1 protein and Atg5 mRNA in the lung tissues were detected by reverse transcription PCR(RT‐PCR) .Microtubules associated protein light chain 3(LC3)in the lung tissues were detected by West‐ern blot test .Results Compared with Sham group ,the level of serum LDH in I/R group were very higher than Sham group(P<0 .01) ,which showed that the rats models of LIR‐ALI were established .The expression of specific antibody ,Beclin1 protein ,Atg5 mRNA and LC3‐Ⅰ ,LC3‐Ⅱ and the ratio of LC3‐Ⅱ /GAPDH in lung tissues were all very higher in I/R group than in Sham group (P<0 .01) .Conclusion ALI can be induced by LIR and the expression of autophagy associated proteins in lung tissues in LIR‐ALI model is very higher in rats .