Comparison of the infected mouse brain by Nakayama and Beijing -1 strain for Japanese Encephalitis vaccine production

ABSTRACT

Using white Swiss mouse at 3-4 weeks of age for injection of Japanese Encephalitis (JE) virus. Every 8 lots of the mouse had been infected with Nakayama and Beijing-1 strains by injection of 0.03ml into their brains, then followed up within 3 days. The paralysed mice were collected at the 69th hour of the beginning and at the 120th hour of the end. The interval between collection time was every 3 hours. In this study, the rate of mice with paralysis due to Beijing-1 strain was higher than those due to Nakayama strain (94.99% and 92.97%, respectively). The authors suggested that use of Beijing-1 strain to produce JE vaccine is more effective than Nakayama strain.