Determination of content and related substances of caffeine chewable tablets by HPLC / 国际药学研究杂志

ABSTRACT

Objective To establish a method for determination of the content and related substances in caffeine chewable tablets. Methods Agilent TC - C18 column(250 mm×4.6 mm;5μm)was used. The mobile phase was methanol- sodium dihydrogen phosphate buffer solution(10mmol/L,adjusted to pH 3.0 with phosphoric acid )(7525,V/V),with a flow rate 1.0 ml/min,and the detection wavelength was 273 nm. The column temperature was 30℃ and the injection volume was 20 μl. Results The calibration curves showed good linearity(r=1,n=7)within ranges of 1-400 μg/ml. The average recovery of the method was about 100.04%(RSD=0.12%,n=9),and the stability of working solutions was acceptable in 24 h (RSD=1.07%,n=8). Conclusion The results indicated that the developed method could be readily utilized as a method for the quality control,including contents determination, intermediate products and stability study.