Determination Optimization of 15 Sun-screening Agents in Cosmetics / 中国药师

ABSTRACT

Objective:To improve and optimize the determination methods for 15 chemical sun-screening agents in cosmetics by HPLC described in Hygienic Standard for Cosmetics (2007 edition) to enhance the quality control of limited chemical sun-screening a-gents in cosmetics. Methods:The samples were extracted using the improved mixed liquor. The detection was performed on an Accur-asilC18(250mm×4.6mm,5um)analyticalcolumn,themobilephasewasmethanol-tetrahydrofuran-water(adjustingpHto2.60 with acetic acid) with gradient elution, the flow rate was 1. 0 ml·min-1 , the column temperature was 25℃,and the detection wave-length was 311nm. Results:The method showed good linear relationship and the correlation coefficient was above 0. 999 5, the detec-tion limit was 1. 9-18. 7 ng, and the average recovery was within the range of 97. 5%-113. 7% with RSD of 0. 85%-1. 56%(n=9). Conclusion:The improved method is reliable, which is the effective optimization and complement for the determination methods of chemical sun-screening agents in cosmetic described in Hygienic Standard for Cosmetics (2007 edition).