Content Determination of Baicalin and Phillyrin in Qinqiao Oral Solutions by HPLC / 中国药师

ABSTRACT

Objective: To establish an HPLC method for the determination of baicalin and phillyrin in Qinqiao oral solutions. Methods:The column was Inertsil ODS-SP(250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-phosphate buffer (11. 5 g ammonium dihydrogen phosphate was dissolved in water, and then 1ml phosphoric acid was added, finally the solution was di-luted to 1 000 ml by water)(75∶25). The flow rate was 1. 0 ml·min-1, the detection wavelength was at 278 nm and the column tem-perature was 25℃. Results:The calibration curve was linear within the range of 0. 203-4. 064 μg(r=0. 999 8) for baicalin and 0. 182-3. 648μg(r=0. 999 7) for phillyrin. The average recovery was 100. 9% with RSD of 1. 7% (n=5) and 100. 5% with RSD of 1. 4% (n=5), respectively. Conclusion:The method is simple, practicable, accurate and rapid, which can be applied in the determi-nation of baicalin and phillyrin in Qinqiao oral solutions.