Invasion and metastasis inhibition of Foxp3 on ovarian cancer cells Skov3 and its mechanism / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 173-175,179, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-600630
ABSTRACT
Objective:
To investigate the effect of Foxp3 on the invasion and metastasis of the ovarian cancer cells Skov 3 and the related mechanism.Methods:
Plasmid overexpressing Foxp 3 gene was constructed and transfected into Skov 3 cells using liposome.Proliferation,invasion,RT-PCR and Western blot analysis were done to observe the expression of Foxp 3 gene in Skov3 cells and the invasion and metastasis ability.Also,MMP-2 and MMP-9 gene expression was detected.Results:
Overexpression of Foxp3 was confirmed in Skov3 cells after Foxp3 plasmid transfection.Compared with the empty vector group , overexpression of Foxp3 gene significantly inhibited the migration and invasion of Skov 3 cells.Meanwhile , the expression level of MMP-2 and MMP-9 genes were decreased.Conclusion:
Upregulation of FoxP3 gene expression can inhibit cell proliferation ,invasion and migration in ovarian cancer Skov3 cells through downregulating MMP-2 and MMP-9 gene expression.This study provided experimental basis for further research on Foxp3 gene and gene therapy for ovarian cancer.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2015
Tipo de documento:
Artigo
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