Inhibit effect of Curcumin on human papilloma virus replication by inducing cell autophagy / 国际检验医学杂志

ABSTRACT

Objective To explore the effect of autophagy induced by Curcumin on human papilloma virus (HPV) replication . Methods Human cervical cancer cell SiHa were treated with Curcumin to induce autophagy .Fluorescence microscope was used to observe the autophagosome .Western blotting was performed to analyze the expression level of autophagy marker protein LC3‐ Ⅱ /LC3‐ Ⅰ .Real time‐polymerase chain reaction(PCR) and Western blotting were used to detect HPV E6 mRNA and protein expres‐sion level .After adding the autophagy inhibitor 3‐methyl adenine (3‐MA) ,real time‐PCR and Western blotting was employed to detect the expression level of HPV E6 .Results The fluorescence intensity of SiHa cells treated by Curcumin was significant in‐creased ,while the ratio of LC3‐ Ⅱ and LC3‐ Ⅰ was also significantly increased (P< 0 .05) .The expression of HPV E6 mRNA and protein were decreased significantly after induction of autophagy ,while increased significantly after adding autophagy inhibitor 3‐MA .Conclusion Curcumin might inhibit HPV replication by inducing cell autophagy .