Macrophage migration inhibitory factor potentially enhance the proliferation of fibroblast induced by lipopolysaccharide through increasing Toll-like recetor 4 / 中华风湿病学杂志

ABSTRACT

Objective Based on the research of the mechanism of fibroblast proliferation,the principle of synovial hyperplasia is explored in rheumatoid arthritis (RA).Methods CCK-8 method was used to observe the stimulation effect of LPS on fibroblast proliferation.By confocal scanning microscopy,the expression of TLR-4 on fibroblast membrane was observed; Real-time fluorescent quantitative PCR method was used to detect the stimulatory effect of macrophage migration inhibitory factor on TLR-4 expression of fibrob-lasts and further analyzed the changes of TLR-4 expression on the cell membrane by flow cytometry.Results The LPS could stimulate fibroblast proliferation,and the fibroblasts expression of TLR4 per se.After stimulated by macrophage migration inhibitory factor,the expression of fibroblast TLR-4 was increased by about 20％-30％.Conclusion MIF increases the TLR-4 expression of fibroblasts that potentially enhance cell proliferation induced by LPS.