The preliminary study of the mechanism of hydrogen therapy in gouty-animal models / 中华风湿病学杂志

ABSTRACT

Objective To investigate the mechanism of hydrogen therapy for gouty arthritis,and provide new strategy for gout via a diet therapy by building up the crystal arthritis (gout) animal model.Methods Wistar rats (200±20) g were randomly divided into five groups which were consisted of five rats,includingthe hydrogen-water model group,the hydrogen-feed model group,the hydrogen-water and hydrogenfeed model group,the control model group,and the blank group.And the first,the second,the third group collectively referred to as the hydrogen group.The rat model of acute gouty arthritis was established via injecting monosodium urate (MSU) in rats' ankles,after 14 days continuous feeding.On the third day after injection,serum samples were collected and analyzed.The swelling feet were removed and kept in the 40％ neutral formaldehyde for histochemicalstudies.During the three days after MSU injection,the volume of whole feet (including the ankle joint) was also recorded.The results were analyzed with one-way ANOVA and tamhane's T2 methods.Results In this study,a moderate elevated level of observed parameters,such as swelling joints numbers and inflammatory factor levels,was observed in the hydrogen feed model group than other groups.To measure the volume of rats' feet and found that the volume of feet of the control model group was 1.24 times (0.40±0.06,P＜0.05) as big as hydrogen group.And the TNF-α,IL-1,malondialdehyde in the serum of the hydrogen group were also less than those of the control model group.The contention of TNF-α of the control model group was 6.23 times (336±60,P＜0.05) as much as the hydrogen group.The contention of IL-1β of the control model group was 4.02 times (249±42,P＜0.05) as much as the hydrogen group.The contention of MDA of the control model group was 2.18 times (24±4,P＜0.05) as much as the hydrogen group.Conclusion The oral hydrogen intake has a definite therapeutic effect on controlling articular inflammation,which is helpful in exploring the dietary therapy for gouty arthritis.