Proliferation activating effects of Mtb-Ag on γδT cells and its effects on expression of CD69 molecules inγδT cells / 国际生物医学工程杂志
International Journal of Biomedical Engineering
; (6): 29-31,35, 2015.
Article
em Zh
| WPRIM
| ID: wpr-601606
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WPRO
ABSTRACT
Objective To activate and amplify γδT cells with low molecular peptide antigen of Mycobacterium tuberculosis low molecular peptide antigen (Mtb-Ag),and to investigate the expression of CD69 molecules on γδT cellular surface.Methods Healthy human peripheral blood mononuclear cells (PBMC) were obtained and separated,then positive cells were isolated by immuno-magnetic beads selection,and the proportion of γδT cells in the PBMCs was detected by fluorescent monoclonal TCR γδT-PE staining and flow cytometry.Expression of CD69 molecules in γδT cells was detected by γδ-PE/CD69 FITC double staining.Results The proportion of γδT cells was (4.9±1.85)% in freshly obtained PBMC,(69.2±6.57)% after 10 d of Mtb-Ag activation,and (99.3±8.92)% after immuno-magnetic beads selection.The expression of CD69 molecules in γδT cells reached the peak (75.2%) at 24 h after initial Mtb-Ag stimulation,and reached the peak (72.0%) at 6 h after second stimulation.Conclusions MtbAg can specifically stimulate the proliferation of γδT cells in the PBMC.Both its initial and the second stimulation can specifically activate γδT cells.
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Idioma:
Zh
Revista:
International Journal of Biomedical Engineering
Ano de publicação:
2015
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Article